Manual

 


X-RaY crystallography FACILITY, Kasha Laboratory

Institute of Molecular Biophysics

Florida State University, Tallahassee, FL 32306-4380

Data Collection & Processing Guide


Macromolecular x-Ray Facility Crystallography Facility

Data Collection & Processing Guide

Copyright ã 2000-2004 Thayumanasamy Somasundaram

410-414 Kasha Laboratory • Institute of Molecular Biophysics

Florida State University, Tallahassee, FL 32306-4380

E-mail: soma@sb.fsu.edu • URL: http://www.sb.fsu.edu/~soma

http://www.sb.fsu.edu/~xray

Phone 850.644.6448 • Fax 850.644.7244

September 28, 2004

 

 




Chapter

1


Facility Information & Contact Numbers

This chapter deals with the Information about the X-Ray Crystallography Facility and the names and contact numbers of relevant people.

Facility Information

t

he X- Ray Facility (XRF) established in 1993 is located on the fourth floor of,  Kasha Laboratory,  Institute of Molecular Biophysics.  It occupies ~1200 ft2 of space spread among four different rooms KLB 410, 411, 412, & 413.  It is a multi user macro-molecular x-ray diffraction laboratory equipped with three copper rotating anode generators (Rigaku H2Rs and Elliott GX-20), one R-Axis IIc image plate (IP) detector and one Mar165 charge-coupled device (CCD) detector.  The x-ray generators are coupled to either Osmic Confocal Max Flux or Supper total reflection double mirror systems.  Automated data collection and detector control are handled by dedicated Windows NT and Linux computers.  X-ray diffraction data can be collected at variety of temperatures including cryo temperatures.  Crystallographers and scientists from the Structural Biology Program at the Florida State University campus and State University System use the facility to collect x-ray diffraction data from single crystals of biological macromolecules.  Dedicated and shared computer resources in several platforms (HP-Alphas, Linux, WinNT, and WinXP) are available for data manipulation & modeling.  Popular software packages like HKL2000, Mosflm, CCP4, X-PLOR, CNS, ‘O’, RsRef, TNT, ShelX, and XtalView running under different operating systems are available.  Data archiving using DDS-4, DDS-3, and DVD±R, ±RW media is supported. The facility has a precession camera and several optical microscopes including an Olympus Stereo Zoom Microscope SZ-6045 with SZX-Ill Illuminator base for crystal mounting purposes and a 1.3 Mega pixel Motic digital camera with a Leica S8 Apochromatic Optic System with Windows 2000 computer. The facility has state of the art low temperature system for collecting cryo crystallographic data consisting of two Oxford Cryosystems'  Cryo Stream cryo coolers and an American Magnetics' auto refill system. The facility has access to a machine shop and an electronics shop. The facility has a low vibration reach-in Crystallization Chamber for growing crystals at temperatures in the range 10-26° C. We have an Environmental Monitoring Unit  for measuring the temperature and relative humidity conditions of a crystal set-up and detector rooms.

SERVICE

The facility provides the following services:

1.       Single crystal (capillary or loop mounted) x-ray diffraction data collection