Crystallization of Biological Macromolecules

Features

• Author: Alexander McPherson.
• Publisher: Cold Spring Harbor Laboratory Press 1999.
• ISBN: 0-87969-527-7.

Description

Crystals of macromolecules have become keystones of molecular biology, bridging recombinant DNA and X-ray diffraction analysis. Protein, nucleic acid, and virus crystal growth are now possible, raising hopes that virtually all macromolecules might be studied in this way. This extensively illustrated book, by a master practitioner, provides a biochemical context in which crystal growth can be pursued and instructs readers in practical aspects of the technology, laying out effective strategies for success. The underlying physical and chemical principles are presented in approachable form and the most recent advances are described, those that have succeeded in transforming the growth of macromolecular crystals from "black art" to a firmly founded science.