Thomas Wendt
- EMBL Heideberg
- Meyerhofstrasse 1
- Germany
- phone: +49 6221-387- 256
- Personal web page
Figure
Legend: Averaged projection of smooth muscle heavy meromyosin in
the dephosphorolated state preserved frozen hydrated in amorphous ice.
2D array was grown on a lipid monolayer.
Smooth muscle heavy meromyosin (HMM) is a truncated double-headed myosin molecule. Two-dimensional crystals bound to a lipid monolayer were examined by cryo-electron microscopy on a 300keV FEG microscope. Data was collected for untilted and tilted specimen and a 2D projection map was calculated giving a resolution of 2.8 nm.
The crystals show unit cell dimensions of 12.9 by 28.1 nm suggesting that there are 2 HMM molecules per unit cell with P2 symmetry.
Publications
- Thomas Wendt, Dianne Taylor, Kathy Trybus, Terri Messier and Kenneth
A. Taylor. Visualization of head-head interactions in the inhibited state
of smooth muscle myosin. J. Cell Biol. 147, 1385-1390 (1999)
- Thomas Wendt, Dianne Taylor, Kathleen M. Trybus, and Kenneth Taylor.
3-D image reconstruction of dephosphorylated smooth muscle heavy meromyosin
reveals asymmetry in the interaction between myosin heads and placement
of subfragment 2. Proc. Natl. Acad. Sci. 98(8), 4361-4366 (2001)
- Jun Liu, Thomas Wendt, Dianne W. Taylor and Kenneth A. Taylor. Refined model of the 10S conformation of smooth muscle myosin by cryoEM 3-D image reconstruction. J. Mol. Biol. 329(5), 963-972 (2003)