Gary Connors

 

My role in the Taylor lab is that of data analysis. I digitize electron micrographs and compute 3-D images from them. The data are tilt series images collected around a single tilt axis by a method known as electron tomography. After digitizing the images, I clean them up and align them by a process known as cross correlation. After the images are aligned and scaled, I compute a 3-D image.

Currently I am working on tomograms of stretch activated insect flight muscle and of Drosophilia flight muscle in the wild type and mutant with a non-phosphorylated light chain. I have just completed a tomogram of Lethocerus flight muscle labeled with myosin subfragment 1.

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