The Logan Group

 

Dr. DaQun Ni, Postdoctoral Fellow


DaQun is working on NMR spectroscopy of glcyoproteins. He is currently developing isotope-enrichment strategies for recombinant proteins produced in mammalian cells, while also developing NMR methods for characterizing the structure and dynamics of intact glycoproteins.
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Dr. Nilakshee Bhattacharya, Postdoctoral Fellow


Nilakshee is investigating the backbone dynamics of an intramolecular complex formed between the SH3 domain and proline-rich peptide segments of DtxR. Her approach is to use heteronuclear NMR methods to obtain 15N R1, R2, and NOE measurements. These data are analyzed using the standard Lipari-Szabo Model-free formalism and by the newer, more model-independent Reduced Spectral Density Formalism. The goal of these studies is to provide insights into the dynamics associated with intramolecular interactions in the inactive, apo state of DtxR.
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Jason Crowe, Molecular Biophysics Graduate Student


Jason is pioneering a new project using NMR spectroscopy to study stem cell metabolism. He has designed a very clever three dimensional bioreactor that will allow us to use 31P and possibly 13C NMR spectroscopy to study metabolism of these cells in vivo. The goal of this research is to use NMR to identify metabolic "states" that allow us to stage the development of human mesenchymal stem cells.
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Josh Kogot, Biochemistry Graduate Student


Josh is co-mentored by Dr. Logan and Dr. Geoff Strouse. Josh's research focuses on the use of gold nano-particles and NSET spectroscopy in solving biological problems. NSET is an improvement on fluorescence resonance energy transfer spectroscopy in which the distance dependence is extended (out to 200 A) and the dependence on Donor - Acceptor overlap integral and orientation are eliminated. We plan to use this spectroscopy to investigate assembly of protein-DNA complexes, in particular of IdeR.
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Kyle Noble, Molecular Biophysics Graduate Student

Kyle joined the lab in Spring of 2006. He is working on the NMR Spectroscopy of glycoproteins. At this time he is learning eukaryotic cell culture, and how to express and purify recombinant glycoproteins. Stay tuned for future developments as he becomes more familiar with the science of glycoprotein structural biology.
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Dave Skelton, Chemistry and Biochemistry Graduate Student


Dave joined the lab in the Spring, 2007. He is being trained in several areas and has not yet decided on a dissertation project.
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Brian Stapleton, Chemistry and Biochemistry Graduate Student


Brian joined the lab in the Spring, 2007. He is currently being trained in several areas and has not yet decided on a dissertation project.
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Larry Walker, Chemistry and Biochemistry Graduate Student


Larry (sorry ladies, this is not actually Larry Walker) joined the lab in the Spring, 2007. He is currently being trained in several areas and has not yet decided on a dissertation project.
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Former Logan Group Members
 

Wendy Walton, Ph.D, Molecular Biophysics, December, 2005


Wendy defended her dissertation on December 9, 2005. Her doctoral research was devoted to optimizing the production and purification of recombinant Thy-1 glycoprotein in Lec1 mammalian and Sf9 or Tn5 insect cells. She has optimized culture procedures, purification protocols, and has worked to identify an economical approach to isotope enrichment of recombinant proteins produced in Lec1 cells. Wendy has accepted a position at Schering-Plough and started at the end of May, 2006. Good luck, Wendy!

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K. Ilker Sen, Ph.D, Molecular Biophysics, January, 2007


Ilker (that's him on the right) used EPR spectroscopy to investigate the activation of the anthracis repressor (AntR), the DtxR homologue from Bacillus anthracis. He has used Mn(II) EPR spectroscopy to determine metal binding energetics and to characterize the nature of the metal binding site. More recently he has been refining methods for determining inter-spin distances via the 4-pulse DEER experiment. He is applying these methods to monitor conformational changes in AntR as a function of metal binding. Ilker defended his dissertation in January, 2007, and is now a postdoc at NYU. Good Luck, Ilker!

 
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Maria Semavina, Ph.D, Molecular Biophysics, April, 2006


Maria investigated the energetics of metal binding and dimerization during the activation of DtxR and IdeR. She has used equilibrium dialysis to determine metal binding energetics. She also used analytical ultracentrifugation to determine dimerization energetics as a function of metal ligation in IdeR. These data are being analyzed to suggest a mechanism for the activation of DtxR and IdeR upon metal binding. Maria is currently a postdoc at Cornel Medical Center. Good Luck, Maria!

 

 
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Sumiko Takahashi, M.S., Chemistry and Biochemistry, May, 2006

Sumiko defended her Master's Thesis in the Spring, 2006. After travelling around for a few months she returned to work in my lab before obtaining a permanent position at Pfizer in Nagoya, Japan, which she started in March, 2007. Good luck, Sumiko!
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Fun Logan Group Pictures

Nilakshee and Sumiko in Traditional Dresses.
Tim and Nancy skiing in Colorado.
 
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