FSU-Structural Biology:
 25 Years of CryoEM of
 Biological Macromolecules
January 8-9, 1999

Turnbull
Conference
Center
555 W. Pensacola St.
Tallahassee, Florida


Organizing Committee:

Donald L. D. Caspar
Florida State University

Wah Chiu
Baylor College of Medicine

Robert M. Glaeser
University of California at Berkeley

Kenneth A. Taylor
Florida State University


It has been 25 years since the first demonstration that frozen hydrated specimens could be used to study the structure of labile biological macromolecules using the electron microscope. This Symposium will examine the present state of the field and its future directions. In the past 25 years, the technique has been applied to 3-D imaging of specimens ranging in size from single protein molecules to large macromolecular complexes including viruses, helical filaments and 2-D and 3-D crystals. The impact of cryoelectron microscopy on biology will be surveyed by authorities in the field who have pioneered this technique. Recent advances in the structural and functional organization of molecular motors, cytoskeleton, ribosomes, viruses and membranes will be discussed by distinguished leaders and younger investigators who are opening new frontiers in cryoelectron microscopy.

  
In the session on molecular motors and the cytoskeleton, analysis of conformational substates in microtubule and actin associated motors, as well as actin complexed with specific binding proteins will be presented. The high resolution structure of tubulin, which will be described, is a dramatic recent achievement in electron crystallography. The session on single particles will explore application of new techniques, including tomography, to determine the structure and interactions of ribosomes, mitochondrial membrane proteins, and icosahedrally symmetric virus particles. Images of ribosomes at 15Å resolution complexes with tRNA are providing insights into mechanisms of protein synthesis, and the 7Å resolution achieved with complex icosahedral viruses that have not been crystallized is illuminating higher levels of viral organization. The cryomicroscopy of two-dimensional crystals, in particular assemblies of membrane proteins, has been a major success story. In this session, new structural analyses nearing completion will be reported and prospects of further advances in 2-D electron crystallography will be discussed.

PROGRAM

Friday, January 8, 1999

8:00 a.m. - Registration Sign In
8:00 a.m. - Coffee and Refreshments

9:00 a.m.
Introduction, Welcome and Expression of Appreciation to the National Institutes of Health and the National Science Foundation for support of the Philips 300 kV Field Emission Gun EM facility

Dr. Susan D. Allen
Vice President for Research
Florida State University

9:15 a.m. - Opening Remarks: Donald L. D. Caspar

Session I - Impact of CryoEM on Biology
Robert M. Glaeser, Chair

9:30 a.m.
"An Introduction: Impact and Uniqueness of the EM Technique"
Prof. Wah Chiu
Baylor College of Medicine, Houston, TX
10:00 a.m.
"Vitrification and Vitreous Water"
Prof. Jaques Dubochet
Universite de Lausanne, Switzerland

10:30 a.m. - Break

11:00 a.m.
"Electron Tomography of Molecules and Cells"
Prof. Dr. Wolfgang Baumeister
Max-Planck-Institut für Biochemie,
Martinsried, Germany
11:30 a.m.
"Cryo-EM of Virues Big and Small, Round and Not So Round, From Beginning to End"
Prof. Timothy S. Baker
Purdue University, West Lafayette, IN

12:00 p.m. - Lunch

Session II - Molecular Motors and Cytoskeleton
Kenneth A. Taylor, Chair

1:00 p.m.
"Structural Studies of Molecular Motors by Cryo-EM"
Dr. Ronald Milligan
Scripps Research Institute, La Jolla, CA
1:30 p.m.
"Coordinated Time-resolved X-ray Diffraction, Mechanics and Three-dimensional Electron Microscopy of Fast-frozen Contracting Insect Flight Muscle"
Prof. Michael Reedy
Duke University Medical Center, Durham, NC
2:00 p.m.
"Electron Cryomicroscopical Studies of Conformational States of Actomyosin"
Dr. Dorit Hanein
Brandeis University, Waltham, MA
2:30 p.m.
"Electron Cryomicroscopy of Cofilin/F-actin: More Twists and Turns"
Dr. Amy McGough
Baylor College of Medicine, Houston, TX

3:00 p.m. - Break

3:30 p.m.
"Tubulin Structure: Insights on Microtubule Functions and Properties"
Dr. Kenneth H. Downing
Ernest Orlando Lawrence Berkeley
National Laboratory, Berkeley, CA
4:00 p.m.
"Nucleotide-dependent Structural Changes in Kinesin and Related Proteins Complexed to Microtubules"
Dr. Keiko Hirose
National Institute for Advanced
Interdisciplinary Research, Ibaraki, Japan

Refreshments and Poster Presentations [MORE]
Saturday, January 9, 1999

8:00 a.m. - Coffee and Refreshments

9:00 a.m. - Keynote Address:
Introduction: Kenneth A. Taylor
"Opportunities in 3-D EM of Macromolecules"
Professor Robert M. Glaeser
University of California at Berkeley

Session III - Single Particles - Viruses
Donald L. D. Caspar, Chair

10:00 a.m.
"Insight into the Ribosome Elongation Cycle by Means of Cryo-electron Microscopy"
Dr. Joachim Frank
Wadsworth Center, Albany, NY

10:30 a.m. - Break

11:00 a.m.
"Electron Microscopy of Mitochondrial Complex I"
Dr. Niko Grigorieff
MRC Laboratory of Molecular Biology,
Cambridge, UK
11:30 a.m.
"Cold Fusion"
Dr. Stephen Fuller
Supercomputing Resource for
Molecular Biology, Heidelberg, Germany
12:00 p.m.
"High Resolution Cryo-microscopy of Hepatitis B Virus Core Protein Shells"
Dr. R. Anthony Crowther
MRC Laboratory of Molecular Biology,
Cambridge, UK

12:30 p.m. - Lunch

Session IV - 2-D Crystals
Wah Chiu, Chair

1:30 p.m.
"Observation of Membrane Protein Structure and Dynamics by Microscopy"
Prof. Andreas Engel
University of Basel, Switzerland
2:00 p.m.
"Projection Structure oh NhaA, a Secondary Transporter from E. coli at 8Å"
Dr. Karen Williams
Max-Planck-Institut für Biophysik
Frankfurt, Germany
2:30 p.m.
"Structure of the Gap Junction Intercellular Channel at 7Å Resoultion - From Maps to Models"
Dr. Vinzenz M. Unger
Max-Planck-Institut für Biophysik
Frankfurt, Germany

3:00 p.m. - Break

3:30 p.m.
"Three-dimensional Map of the P-type Ion Pump H+-ATPase at 8Å Resolution"
Dr. Manfred Auer
New York University Medical Center, NY
4:00 p.m.
"Binding of Annexins to Membranes: The Contribution of Cryo-EM"
Prof. Alain Brisson
University of Groningen, The Netherlands
4:30 p.m.
"The Use of Lipid Monolayers for Two-dimensional Crystallization of Cytoskeleton Proteins and for Two-dimensional Assembly of Cytoskeleton Complexes"
Prof. Kenneth A. Taylor
Florida State University, Tallahassee, FL

5:00 p.m. - Closing Remarks
Prof. Kenneth A. Taylor


POSTER ABSTRACTS

NEWS RELEASE

MAP OF TALLAHASSEE


Cryo-EM Symposium
Florida State University
Institute of Molecular Biophysics
Tallahassee, FL 32306-4380


The Philips 300 kV FEG EM at
Florida State University


Original: 13 January 1999Updated: June 18, 2008 by TS