IMB Protein Expression Facility

E. coli Protein Expression Workshop:

Web links for protocols and background of techniques

• pET vector and induction of protein expression
• Self-inducing medium for bacteria
• Affinity chromatography
• Nickel slurry for gravity column
• His 60 superflow gravity columns
• SDS-Polyacrylamide Gel Electrophoresis APPENDIX 3M (SDS-PAGE) of Proteins
• Novex Sharp Protein Standardd
• Gel Filtration -separation by molecular weight