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Kasha Laboratory, MC4380
Inst Molecular Biophysics
M-F, 8 AM - 5 PM
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Quench-Flow System

 

 

Quench-flow background

The chemical quench-flow method is used in transient-state kinetic analyses of reactions that cannot be monitored spectroscopically. In a typical experiment, reactants are allowed to age for a finite time, after which the reaction is terminated by addition of a chemical quench solution. The catalytic product is then analyzed by other methods (HPLC or mass spectrometry, for example), leading to determination of the kinetic parameters of the reaction.

KinTek RQF-3 quench-flow system

The KinTek RQF-3 stopped-flow system allows for characterization of kinetic reactions on a 2-100 millisecond time scale. The instrument utilizes a computer-controlled stepper motor and an 8-way reaction valve to achieve the desired reaction time. Temperature control is provided by a circulating water bath.

Vendor

KinTek Corporation

Physical Biochemistry Facility

A Core Facility of the Structural Biology Program, established 1993.

Housed in rm KLB 311-317
Kasha Laboratory Building
91 Chieftan Way
Florida State University
Tallahassee, FL 32306-4380
Phone 850-644-5147

 

Facility Director
Claudius Mundoma, Ph.D., MBA.
850-644-5147
cmundoma@fsu.edu

 

 

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