Kasha Laboratory | Institute
of Molecular Biophysics
Differential Scanning Calorimeter
Differential scanning calorimetry (DSC) is used to obtain thermodynamic parameters associated with heat-induced macromolecular transitions. The apparent molar heat capacity of a macromolecule is measured as a function of temperature, subsequently yielding:
1. the absolute partial heat capacity of a molecule
2. thermodynamic parameters, including change in enthalpy (ΔH), entropy (ΔS), and heat capacity (ΔCp) of the transition
3. the distribution and thermodynamic parameters of intermediate states.
DSC is typically applied to examine stability and domain structure of proteins and nucleic acids, temperature-induced transitions of lipids/membrane systems, and half-life of low molecular weight compounds. For example, proteins may be characterized in the context of mutagenesis, providing information on effects of a specific mutation on protein stability, folding intermediates, and oligomerization.
Microcal VP-DSC calorimeter
The VP-DSC instrument is highly sensitive, allowing for analysis of dilute macromolecular solutions. For example, lysozyme can be analyzed at micromolar concentrations in a 0.5 mL volume. The accessible temperature range is -10oC to 130oC.
The MicroCal VP-DSC is also equipped with a pressure accessory, expanding the instrument’s capabilities into a new area of investigation – pressure perturbation calorimetry. By recording changes in heat as a function of changing pressure above the solution, information on macromolecular solvation, accessible surface area, and solvent structure can be obtained.
Physical Biochemistry Facility
A Core Facility of the Structural Biology Program, established 1993.Housed in rm KLB 311-317
Kasha Laboratory Building
91 Chieftan Way
Florida State University
Tallahassee, FL 32306-4380