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The multi-user macromolecular x-ray crystallography facility (XRF) at the Kasha Laboratory Building (KLB) in the Institute of Molecular Biophysics (IMB) provides the instruments and expertise for collecting and processing x-ray diffraction data from macromolecular single crystals. It is one of the Core Facilities available to all the members of Structural Biology Program. XRF is located on the east side of the fourth floor of the KLB. XRF's floor plan comprises of a 500 sq.ft. Detector Room (KLB 413), a 180 sq.ft. Control Room (KLB 410A), a 160 sq.ft. Crystallization Coldroom (KLB 411), and a 275 sq.ft. Crystal Room (KLB 412). The Detector Room houses two rotating anode generators, two detector systems, cryo and crystal manipulation accessories. The Control Room houses Linux and Windows computers used both for detector control and data processing. Crystallization Coldroom and Crystal Room are dedicated exclusively for crystal set-up, examination, documentation, and mounting purposes.

One (Rigaku RU-H3R) generator is equipped with an Osmic confocal mirror system, a Rigaku Image plate detector (R-Axis IV++), a Rigaku Inverse Phi, and an Oxford CryoStream cooler. This system allows macromolecular x-ray diffraction data collection under wide range of sample-detector distances (100 to 360 mm) and temperatures (100-400K). The data collection and processing is controlled by a dedicated Dell PC running Windows XPSP23 computer. The second (Rigaku RU-H2R) generator is equipped with an Osmic confocal mirror system, a Mar-USA (MarCCD165) detector, and an Oxford CryoStream cooler. This system allows very rapid data collection (read time of 3 seconds) under wide range of sample-detector distances (35 to 325 mm) and range of temperatures (100 to 400K). Data collection and processing from this generator is controlled by a dedicated Pentium III PC running under Red Hat Linux computer

The facility has a stereo-zoom microscope with digital documentation system, several optical microscopes and a low-vibration crystallization chamber for growing crystals at temperatures other than ambient and 4C. Popular x-ray diffraction data processing software suites such as HKL2000, Mosflm, CrystalClear, CCP4, CNS, "O", etc., under different platforms are licensed and available for the users at the Facility. The Facility Director coordinates additional data collection work at the third generation synchrotron x-ray source using FSU's membership in SER-CAT Beamline 22 in Advanced Photon Source.

Starting in July 2010, XRF has instituted User Fees to partially off-set the operational costs of running the Facility. From October 2009, XRF has started to offer Protein Crystal Screening Service.

X-Ray Facility, 410-413 Kasha Laboratory, Institute of Molecular Biophysics
Florida State University, Tallahassee, FL 32306-4380 | (850) 645-1333
The webpage design © FSU & contents © 2003-14 Thayumanasamy Somasundaram.
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